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e coli destination vector pdest 566  (Addgene inc)


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    Structured Review

    Addgene inc e coli destination vector pdest 566
    Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.
    E Coli Destination Vector Pdest 566, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+destination+vector+pdest+566/pDest-566+(Plasmid+%2311517)/pmc06983858-182-31-39
    Average 95 stars, based on 54 article reviews
    e coli destination vector pdest 566 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer"

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.RA119.010934

    Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.
    Figure Legend Snippet: Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.

    Techniques Used: Purification, Construct, Expressing, Solubility

    Related Articles

    Expressing:

    Article Title: Biochemical and structural analysis reveals the Neurofibromin (NF1) protein forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag), or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His 6 tag) and pDest-636 (pFastBac1 with N-terminal His 6 -MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed intoDH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).

    Clone Assay:

    Article Title: Biochemical and structural analysis reveals the Neurofibromin (NF1) protein forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag), or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His 6 tag) and pDest-636 (pFastBac1 with N-terminal His 6 -MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed intoDH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).

    Plasmid Preparation:

    Article Title: Biochemical and structural analysis reveals the Neurofibromin (NF1) protein forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag), or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene #11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (ThermoFisher), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His 6 tag) and pDest-636 (pFastBac1 with N-terminal His 6 -MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR ( ).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed intoDH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer
    Article Snippet: .. Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His6 tag) and pDest-636 (pFastBac1 with N-terminal His6-MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517). .. Final baculovirus expression clones were transformed into DH10Bac cells (Thermo Fisher Scientific), and bacmid clones were verified by PCR (29).



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    e coli destination vector pdest 566  (Addgene inc)
    95
    Addgene inc e coli destination vector pdest 566
    Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.
    E Coli Destination Vector Pdest 566, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+destination+vector+pdest+566/pDest-566+(Plasmid+%2311517)/pmc06983858-182-31-39
    Average 95 stars, based on 1 article reviews
    e coli destination vector pdest 566 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.

    Journal: The Journal of Biological Chemistry

    Article Title: Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer

    doi: 10.1074/jbc.RA119.010934

    Figure Lengend Snippet: Neurofibromin proteins used in this study Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His 6 -MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His 6 -tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.

    Article Snippet: Gateway LR recombination was used to generate final expression clones in baculovirus Destination vectors pDest-635 (pFastBac1 with N-terminal His 6 tag) and pDest-636 (pFastBac1 with N-terminal His 6 -MBP tag) or E. coli Destination vector pDest-566 (N-terminal His6-MBP tag, Addgene catalog no. 11517).

    Techniques: Purification, Construct, Expressing, Solubility